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Figure 1
(a) Density ρ (g ml−1) versus CPA concentration [%(w/w)] near room temperature. Data for methanol, ethanol and 2-propanol (Herráez & Belda, 2006BB17), glycerol (Bosart & Snoddy, 1928BB4; Cristancho et al., 2011BB8), ethylene glycol and PEG 200 (Rahbari-Sisakht et al., 2003BB35; Muller & Rasmussen, 1991BB31), and PPG 425 (Zafarani-Moattar & Salabat, 1998BB56) were obtained from the cited literature. Data were measured at T = 298 K except for those for PEG 200, which were measured at 313 K. The solid lines are fourth-order polynomial fits with the coefficients given in Table 1[link]. (b) Electron density (e Å−3) calculated from the densities in (a), equation (1)[link] and Table 3[link]. (c) Normalized forward scattering from protein versus CPA concentration, calculated using (2)[link]. The corresponding results for nucleic acids are shown in Supplementary Fig. S2(a). (d) Excess specific volume vE versus CPA concentration, calculated using (3)[link] and (4)[link] from the densities in (a).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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