Figure 1
Data analysis and molecular-replacement solution of C-DCX. (a) Stereographic projection of the κ = 180° section of the self-rotation function. The function was calculated over the full resolution range and a sphere radius of 32 Å using POLARRFN (Winn et al., 2011). An additional peak (ω = 90, φ = 22.5°), 69% of the height compared with the expected positions for 422 symmetry, is marked by a red arrow. (b) The four protomers in the asymmetric unit of space group P43212, shown as differently coloured ribbons, form two dimers. The final, domain-swapped structures of the protomers are shown. The view is a projection into the ab plane of the unit cell. The NCS axes of the dimers are shown as black lines. They make an angle of 45° with and are parallel to the crystallographic axes of this space group. The two dimers are related by another twofold axis (red line), which makes an angle of 22.5° with the b axis, explaining the single additional peak in the self-rotation function in (a). |