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Figure 5
Extensive rebuilding yields minimal changes in correlation to the map. Starting from the original model (blue), real-space refinement with the current version of phenix.real_space_refine reduced the overall masked FSC from 0.828 to 0.816, with some improvement in geometry. Rebuilding in ISOLDE followed by a tightly restrained phenix.real_space_refine run as described (black) increased the FSC to 0.821, while running phenix.real_space_refine with default settings on the result (green) further increased the FSC to 0.830 with a slight degradation in geometry. These changes are far smaller than those arising from choice of map sharpening or inclusion/exclusion of H atoms, for example. The vertical dotted line denotes the published resolution of the map.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 74| Part 6| June 2018| Pages 519-530
https://doi.org/10.1107/S2059798318002425
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