issue contents

Journal logoSTRUCTURAL
ISSN: 2059-7983

June 2018 issue

Proceedings of the third CCP-EM Spring Symposium

Edited by Tom Burnley, Paula da Fonseca and Randy Read

Highlighted illustration

Cover illustration: Cryo-EM can now be used to obtain high-resolution structural information on a diverse range of biological specimens. However, for many single particle cryo-EM projects, a major bottleneck remains at the sample preparation stage, where issues such as specimen aggregation, preferred orientation and affinity for the support film prevent the collection of high quality cryo-EM data (see Drulyte et al., p. 560, for details). This cover image highlights the effects of the sample displaying a preferred orientation (right) which results in an under-sampling of some Fourier components and the reduction of overall resolution within the final reconstruction, compared with a more evenly orientated specimen (left) producing a final reconstruction with higher resolution.


link to html
An introduction to the proceedings of the third CCP-EM Spring Symposium.

research papers

link to html
REFMAC5 and related tools for the refinement of atomic models into cryo-EM reconstructions in CCP-EM are reviewed. An upper bound on the correlation between observed and calculated Fourier coefficients is identified, and the practical utility of map blurring/sharpening is discussed. The Divide and Conquer pipeline for refining large complexes in parallel, and ProSHADE for the identification of symmetries in a given map or coordinate set, are presented.

link to html
Adaptations to the DIALS package are described that make it a suitable choice for processing challenging continuous-rotation electron diffraction data. The results of using the extended package are presented for a case consisting of seven example data sets.

link to html
ISOLDE is an interactive molecular-dynamics environment for rebuilding models against experimental cryo-EM or crystallographic maps. Analysis of its results reinforces the need for great care when validating models built into low-resolution data.

link to html
A description is provided of the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite and its application to the re-refinement of cryo-EM-derived models.

link to html
A procedure for optimizing the sharpening of a map based on maximizing the level of detail and connectivity of the map has been developed and applied to 361 pairs of deposited cryo-EM maps and associated models.

link to html
This paper describes different approaches that cryo-EM users can take to improve the quality of their sample distribution and ice for high-resolution single-particle cryo-EM.

link to html
Electron microscopy is a key methodology for studying microtubule structure and organization. Here, the results of cryo-electron tomography experiments on in vitro-polymerized microtubules and comparisons with microtubule ultrastructure in cells are described.

link to html
The use of high-throughput in situ electron cryotomography and subtomogram averaging to study the the architecture and diversity of the bacterial flagellar motor is reviewed. Together with phenotypic analysis, this information can be used to better understand the evolution of molecular machines.
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds