Automated map sharpening by maximization of detail and connectivity

Terwilliger, T.C.; Sobolev, O.V.; Afonine, P.V.; Adams, P.D.

doi:10.1107/S2059798318004655

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 2
Sharpened/blurred versions of the cryo-EM map of the anthrax protective antigen pore (Jiang et al., 2015

). (a) Overall B_iso = −100 Å². (b) Overall B_iso = 60 Å². (c) Overall B_iso = 150 Å². (d) Original map (EMD-6224). (e) Auto-sharpened map, overall B_iso = 20 Å². All contours are drawn at 3σ. Maps were drawn with Coot (Emsley et al., 2010