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Figure 6
Cryo-EM map of the TRPV1 channel (EMD-5778 and PDB entry 3j5p; Liao et al., 2013BB17) in the vicinity of residues 449–455 after the application of sharpening. Contours are chosen to yield equal volumes inside contours in each map (Urzhumtsev et al., 2014BB31). The final values of overall Biso and the contour levels used are as follows: (a) 0 Å2, 1.19σ; (b) 20 Å2, 1.20σ; (c) 40 Å2, 1.24σ; (d) 60 Å2, 1.30σ; (e) 80 Å2, 1.36σ; (f) 100 Å2, 1.41σ; (g) 120 Å2, 1.49σ. Maps were drawn with Coot (Emsley et al., 2010BB9) and RASTER3D (Merritt & Bacon, 1997BB21).

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