Figure 1
Schematic representations of macromolecular complexes distributed in a vitreous ice layer. Top panels, views from the top; bottom panels, views from the side. (a) Ideal vitrified sample exhibiting well dispersed particles adopting random particle orientations. (b) Thinning of the ice in the centre of the hole pushes particles towards the carbon edge, excluding any from the middle and causing particle aggregation. (c) Specimen exhibits high affinity for the support and is excluded from the holes. (d) Particles adopt a preferential orientation. |