Figure 3
The use of continuous support films. (a) Holey grid showing few virus particles in the vitrified ice. (b) Continuous carbon grid prepared with the same concentration of virus as in (a), showing a drastic increase in the number of virus particles observed. (c) Representative micrograph of a holey grid showing the extreme preferred orientation of β-galactosidase particles. (d) Representative micrograph of a continuous graphene oxide grid with a significantly improved angular distribution of β-galactosidase particles. Scale bars: (a), (b) 200 nm; (c), (d) 50 nm. |