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![[Figure 6]](id5005fig6mag.jpg)
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Figure 6
Characterization of MTs in cultured mouse neurons. (a) Phase-contrast light-microscope overview of mouse cortical neurons growing on cryo-EM grids. (b) Top, 0° tilt image (motion-corrected movie sum; 2.7 e− Å−1 total dose) showing a view of the periphery of a neuron lying across a hole in the cryo-EM grid carbon layer, in which numerous cellular components, including MTs (four are labelled i–iv), are visible. Bottom, 8 nm section through the three-dimensional tomographic reconstruction of the same sample, showing the appearance of sections through the MT wall (red arrowheads), the MT lumen (orange arrowheads), MT ends (pink arrows), actin filaments (green arrows) and membranous organelles (black arrows). (c) Top, Fourier filtered images of the selected four MTs in (a) are shown, which reveal the unvarying moiré pattern typical of 13PF MTs seen in all neuronal MTs examined (n = 61). For a comparison with the PF distribution of in vitro-polymerized mouse brain tubulin, see Vemu et al. (2017  ). |
![[Figure 6]](id5005fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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