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![[Figure 1]](rr5162fig1mag.jpg)
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Figure 1
The effect of map blurring on cryo-EM reconstructions. Electrostatic potential maps and atomic models corresponding to the structure of β-galactosidase (PDB entry 5a1a, EMDB entry EMD-2084; Bartesaghi et al., 2015  ). (a) The original map is very noisy, suggesting that it is over-sharpened. (b) Blurring the map using a B value of 40 Å reduces the noise, appearing to make it largely disappear, although the features (signal) in the map remain clear. This produces a clearer map that it will be easier to refine the atomic model against. (c) Applying further blurring (B value of 100 Å) does not help: it causes more of the noise to disappear, but also causes structural details to be lost (for example regarding side chains). |
![[Figure 1]](rr5162fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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