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![[Figure 3]](rr5162fig3mag.jpg)
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Figure 3
The utility of simultaneously viewing multiple maps using various levels of map blurring/sharpening. Electrostatic potential maps and atomic models corresponding to β-galactosidase (PDB entry 5a1a, EMDB entry EMD-2084; Bartesaghi et al., 2015  ) using different levels of map blurring. (a) In the original/default map, noise is visible in the solvent regions and there is little backbone density visible in the exposed region around residue 732 in chain A. (b) Blurring the map using a B value of 40 Å reduces noise and reveals features in the map. This suggests incorrect modelling of the backbone in this region and indicates where the model backbone should be positioned. (c) Simultaneously viewing an array of maps with different levels of sharpening and blurring further increases map interpretability. Here, maps corresponding to blurring B values of 0, 20, 40, 60, 80 and 100 Å are displayed. In this case, it is evident how the backbone should be traced (from the more blurred maps), and at the same time it is possible to interpret finer structural details such as the orientations of side chains (from the sharper maps). |
![[Figure 3]](rr5162fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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