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![[Figure 2]](mn5115fig2mag.jpg)
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Figure 2
(a) A cryocooled perdeuterated crystal of Toho1 β-lactamase in complex with the antibiotic cefotaxime, freeze-trapping a short-lived intermediate over a 40 h data-collection time. The crystal volume is around 0.5 mm3 and it is mounted in a specially made 2 mm diameter litholoop from Molecular Dimensions. The large crystal fits snugly into the custom-sized loop, making it less likely that the crystal will be lost during the cryocooling process. (b) A time-of-flight wavelength-resolved slice from one of five 8 h diffraction images from a Toho1 β-lactamase data collection on the MaNDi instrument. |
![[Figure 2]](mn5115fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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