Figure 1
Three-dimensional structure of the Alicyclobacillus α-amylase AliC. (a) Three-dimensional protein cartoon, coloured by domain, with metal ions shown as shaded spheres and the acarbose and the +2′ glucose shown as van der Waals spheres. (b) Electron density for the transglycosylated acarbose in subsites −4 to +2 (and +2′ Glc) binding; density for an isolated `+3' glucose is not shown. (c) Electron density for the binding of the branched oligosaccharide in subsites +1, +1 and +1′. Electron-density maps are REFMAC maximum-likelihood-weighted 2Fo − Fc syntheses contoured at 1σ. This figure was drawn with CCP4mg (McNicholas et al., 2011). |