The structure of the AliC GH13 α-amylase from Ali­cyclobacillus sp. reveals the accommodation of starch branching points in the α-amylase family

In the light of NMR data on product profiles, the structure of an Alicyclobacillus sp. CAZy family GH13 α-amylase highlights the accommodation of branch points in the α-amylase active centre.

Structure and oligomerization state of the C-terminal region of the Middle East respiratory syndrome coronavirus nucleoprotein

The X-ray structure and SAXS analysis of the C-terminal domain of the nucleocapsid from Middle East respiratory syndrome coronavirus, an emerging virus, are reported.

Iron–sulfur clusters have no right angles

A set of restraints for an iron–sulfur cluster based on small-molecule structures was generated and tested in structure refinement. Additionally, the small-molecule structures also provided bond and angle restraints for linking the cluster to the coordinating cysteine residues.

Structure of interferon-stimulated gene product 15 (ISG15) from the bat species Myotis davidii and the impact of interdomain ISG15 interactions on viral protein engagement

The structure of interferon-stimulated gene product 15 (ISG15) from the bat species M. davidii was elucidated to 1.37 Å resolution. Structural comparison of this ISG15 originating from a species of vesper bat endemic to China with those from mouse and human illuminates a conserved hydrophobic interface between the ubiquitin-like domains of ISG15. The importance of this interaction was probed for its influence on ISG15 engagement by a papain-like viral deISGylase using isothermal titration calorimetry.

Utilizing guanine-coordinated Zn²⁺ ions to determine DNA crystal structures by single-wavelength anomalous diffraction

Guanine N7–Zn²⁺ coordination served as the basis for the determination of three DNA crystal structures using zinc single-wavelength anomalous diffraction. This method is likely to be broadly applicable to the experimental determination of crystal structures of nucleic acids.

Biochemical characterization of Mycobacterium tuberculosis LexA and structural studies of its C-terminal segment

LexA, a protein that is involved in the SOS response, from Mycobacterium tuberculosis and its mutants have been biochemically characterized and the structures of their catalytic segments have been determined.

Crystal structures of the complex of a kallikrein inhibitor from Bauhinia bauhinioides with trypsin and modeling of kallikrein complexes

Crystal structures of the complex of a serine protease inhibitor from Bauhinia bauhinioides with bovine trypsin were determined in two crystal forms, which served as templates for modeling the structures of its complexes with plasma and tissue kallikreins.

Structural studies of a surface-entropy reduction mutant of O-GlcNAcase

The surface-entropy reduction method has been used to generate new crystal forms of human O-GlcNAcase.

Mask-based approach to phasing of single-particle diffraction data. II. Likelihood-based selection criteria

A new likelihood-based estimate of the quality of randomly generated molecular masks has been suggested and tested in a mask-based phasing procedure that was applied to simulated data for the photosystem II single particle. Using this selection criterion led to phase values that had 98% correlation with the true values for all 4000 reflections with resolution below 16 Å.

The crystal structure of the N-acetylglucosamine 2-epimerase from Nostoc sp. KVJ10 reveals the true dimer

The N-acetylglucosamine 2-epimerase (AGE) from Nostoc sp. KVJ10 (nAGE10) was crystallized in a different space group to other AGEs. The nAGE10 dimer, while different from the proposed AGE dimers in previously published structures, can also be found in these structures and is probably the biological dimer.

Crystal structure of Mycobacterium tuberculosis FadB2 implicated in mycobacterial β-oxidation

The catabolism of fatty acids in mycobacteria involves an extensively redundant set of enzymes. Here, the structure of the L-3-hydroxyacyl-CoA dehydrogenase FadB2 is described and structural cues as to how it may work with other components of β-oxidation are explored.

Biological Small-Angle Scattering: Theory and Practice