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Figure 4
Interpretation of the limit digest patterns in terms of protein structure. Black and red arrows indicate the two/three places at which bonds must be cut to accommodate the limit dextrins observed. To generate panose from pullulan, both the +1 and −2 subsites must accommodate α-1,6 branches. The branched-ligand complex shows how subsite +1 can accommodate a branch (Fig. 1c), and in subsite −2 the O6 of acarbose was solvent-exposed. To generate the branched limit dextrin from amylopectin (Fig. 3[link]), AliC must also be able to accommodate branching in the +2 subsite, which is consistent with the glucose moiety seen adjacent to O6 of the +2 sugar (Fig. 1[link]b).

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ISSN: 2059-7983
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