Figure 2
(a, b) Crystal symmetry of HsOGAE602AE605A. (a) The connections made between the dimers show the trigonal solvent channel, with 50% solvent as calculated from the Matthews coefficient of 2.51 Å3 Da−1 (Kantardjieff & Rupp, 2003; Matthews, 1968). (b) Side view of the repeating trigonal dimers showing the linking C-terminus of monomer A binding into monomer A of the next dimer complex. The monomers are labelled A and B, with asterisks indicating the C-termini. (c, d) Disordered loop regions were stabilized in the new crystal form. Monomer A is shown in sea green and monomer B in brown. (c) The regions in purple were built into the HsOGAE602AE605A structure using PDB entry 5m7r as the template model and were missing from the wild-type structure. (d) Residues Lys341–Gly347 and the maximum-likelihood/σA-weighted 2Fobs − Fcalc map shown in red contoured at 0.12 e Å−3. (e) Overlap of the N-terminal monomers A from chain A for both HsOGA11–396+535–715 (monomer A, blue; monomer B, gold) and HsOGAE602AE605A (monomer A, sea green; monomer B, brown) as calculated by CCP4mg Superpose models. The residue range selected for superposition was Arg59/A–His395/A. |