Figure 4
Structure of the active site of nAGE10. nAGE10 is shown in a cartoon representation and is coloured in a green–blue palette from the N-terminus to the C-terminus. Ethylene glycol (yellow) and active-site residues are shown as sticks, and waters and the chloride ion are shown as nonbonded spheres. (a) Active site. Electron density (2Fo − Fc map) is shown around the waters, the chloride ion and the ethylene glycol molecule. Polar contacts for ethylene glycol are coloured black and coordination of the chloride ion is shown in green. (b) Coordination geometry for the buried chloride ion. The five coordinating atoms form a trigonal bipyramid (magenta) with the chloride at the centre of mass (magenta star). |