Figure 5
Comparison of active sites in the AGE family. The active sites of RmCE in complex with cellobiitol (wheat; PDB entry 3wki) and SeYihS in complex with mannose (light pink; PDB entry 2zbl) are superimposed onto the structure of nAGE10 (blues, with ethylene glycol in yellow). The C2 atoms of the mannose rings of cellobiitol and mannose are indicated by black arrows. Residues of nAGE10 are labelled. (a) Overview. Perspective was added for visualization purposes. (b) Detail of the catalytic residues and the interaction with Trp368. (c) Detail of the N-acetyl group binding pocket. |