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![[Figure 7]](jc5019fig7mag.jpg)
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Figure 7
Synthesis of Neu5Ac by coupled epimerase–aldolase reactions. (a) Reaction scheme for the coupled reaction. Each reaction is performed using either GlcNAc or ManNAc as a substrate. 1: the reaction is performed in the absence of AsNAL and thus only the epimerization reaction occurs. 2: the reaction is performed in the absence of AGE and thus only the condensation reaction occurs. 3: the reaction is performed in the absence of ATP, affecting the epimerization reaction. 4: complete coupled reaction. (b) Detection of Neu5Ac by the TBA assay for reactions 1–4, using either GlcNAc (grey) or ManNAc (black) as a substrate. Production of Neu5Ac is expressed as percentage of the amount produced for the condensation reaction alone (2) using ManNAc as a substrate (grey bar). The reactions were performed at room temperature (25°C) with an incubation time of 1 h. |
![[Figure 7]](jc5019fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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