Figure 2
rMMS can generate new conditions that support the crystal growth of HEWL in the presence of I3C. (a) Crystallization conditions supporting the crystal growth of HEWL in the Index HT screen are shaded in red. Seed stock was made from lysozyme crystals grown in 0.2 M ammonium tartrate dibasic pH 7.0, 20%(w/v) polyethylene glycol 3350. (b) Representative photographs showing crystals that formed with and without seeding both in the presence and absence of I3C. (c) Dilution of seed stock was used to reduce the number of seeds to influence the crystal size. Seed stock was diluted 1:10 to give fewer and larger crystals in condition B12 of Index HT. |