Figure 4
Structure solution of HEWL. (a) The crystal structure of HEWL. (b) Stereoview of a composite OMIT 2mFo − DFc electron-density map of HEWL. The contour level was set to 1σ. (c) Substructure density of each I3C molecule. The anomalous difference map is contoured at 5σ. The map was generated using phenix.maps. (d) The crystal structure of HEWL (PDB entry 6pbb) was superimposed onto a previously solved structure of lysozyme soaked with I3C (PDB entry 3e3d) and shows that two I3C molecules are bound in the same position. |