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![[Figure 1]](ic5110fig1mag.jpg)
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Figure 1
Concept of the initial model-generation procedure. (a) A 3D helical structure consists of an image of a chicken in the XY direction and is devoid of features in the Z direction. (b) The XY cross-section of the helix shown in (a). Grey dashed lines represent a 1D projection operation, which results in a single array of pixels at the bottom of the panel. (c) This array of pixels is inserted as a single column in the image. Repeating the 1D projection operation for 360° yields a so-called sinogram image, which is shown. The initial model-generation procedure outlined in the main text basically performs the inverse of this procedure: reference-free 2D class averages are aligned and summed together to form segments of a complete sinogram image, and a 2D reconstruction is performed with all pixel columns. The resulting 2D image is then inserted as multiple XY slices with varying rotations in the 3D map. |
![[Figure 1]](ic5110fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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