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![[Figure 2]](ic5110fig2mag.jpg)
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Figure 2
Initial model generation from a single 2D class average that spanned an entire cross-over. (a) The 2D class average used for initial model generation. (b) The sinogram covering 360° of rotation along the helical axis, as output by RELION. Note that a single cross-over spans 180°. Therefore, the image in (a) appears twice: one as is and once mirrored in the horizontal axis. (c) The 2D reconstruction obtained from (half of) the pixel columns in (b). (d) Four central slices of the symmetrized, post-processing map after 3D auto-refinement, which used the initial model that was generated from the image in (c). Areas that are too white are highlighted with white arrows and areas that are too black are highlighted with black arrows. (e) Thresholded view of the symmetrized, post-processing map in a view along the helical axis. Unexpected breaks in the density are highlighted with arrows. (f) Thresholded view of the symmetrized, post-processing map in a view perpendicular to the helical axis. Unexpected connections between rungs along the helical axis are highlighted with arrows. |
![[Figure 2]](ic5110fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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