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Figure 3
Initial model generation from multiple 2D class averages in smaller boxes. (a) Three representatives of the 24 2D class averages that were used for the initial model generation. (b) The 2D reconstruction obtained from (half of) the pixel columns in (c). (c) The sinogram covering 360° of rotation along the helical axis, as output by RELION. (d) Four central slices of the symmetrized, post-processing map after two rounds of 3D auto-refinement, which started from the initial model that was generated from the image in (b).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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