Figure 4
Comparisons with the published map. (a) Overlay of the published map (transparent light grey) and the map after refinement of the initial model that was generated from a single 2D class average that spanned an entire cross-over (dark grey) in a view along the helical axis. (b) As in (a), but in a view perpendicular to the helical axis. (c) Enlargement of the boxed region in (a), with an arrow indicating an apparent main-chain break in the dark-grey map. (d) Enlargement of the boxed region in (b), illustrating the different heights of β-strands in the two maps. (e) As in (a) but overlaying the published map (transparent light grey) and the map after two rounds of refinement, which started from the initial model that was generated from multiple aligned 2D class averages in smaller boxes. (f) As in (c) but in a view perpendicular to the helical axis. |