Figure 4
(a) The NCS-constrained electron-density map with dummy atoms that are fully dispersed from the actual atomic positions (yellow sticks) contains the correct electron-density information. (b) The NCS-constrained map with the main-chain trace of the initial model of GNNV (purple) and the final structural model of PvNV-Sd (yellow sticks) fitted to the map as a guide for the eye. Using the NCS-constrained map, the structure model was built. The bias from the initial MR model can be removed. The contour level of the map is 2.3σ. |