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![[Figure 7]](ji5009fig7mag.jpg)
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Figure 7
Comparisons of the DDO map (blue mesh) with the map resulting from REFMAC5 restrained refinement (green mesh). The REFMAC5 map (green mesh) is the weighted (2Fo − Fc) map. The contour levels of both maps are 2.0σ. Both maps were calculated using the 3.37 Å resolution data at the phasing stage and the initial NCS matrices which were obtained from the MR solution. Yellow carbon bonds are the final model fitted to the NCS-constrained map. Brown carbon bonds are the result of REFMAC5 restrained refinement. Cα displacements between the final fitted model and the MR solution are 2.7 Å on average. Most of the refined main chains (brown) jumped to fit to the new position, as shown in (a). However, some parts of the refined main chains failed to fit the new map, as shown at the centre of (b). Even though both maps were calculated using the same initial structure, the NCS-constrained map from DDO is free from bias from the initial structure. The yellow stick model is the final model fitted to the map as a guide for the eye. |
![[Figure 7]](ji5009fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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