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Figure 1
Structure solution of MltC (PDB entry 4c5f) using SEQUENCE SLIDER. (a) The final structure is shown in transparent green with a dashed line separating the two monomers. The molecular-replacement solution with the pruned MltE model (PDB entry 2y8p) corresponding to the C-terminal domain of MltC is shown in orange. (b) New fragments of the N-terminal domain obtained from the expansion of the refined solution after C-terminal side chains have been modelled and refined. (c) Further extension of the fragments in the N-terminal domain shown as yellow cartoons, derived from NCS. Related chains are marked with the same number of primes: L, H′, I′′ and O′′′ are equivalent to M, P′, Q′′ and G′′′, respectively. (d) The trace upon SEQUENCE SLIDER side-chain identification of sheets L and M allows the extension of the helices displayed in blue. From this point, SEQUENCE SLIDER can establish the sequence of helices H′ and P′, followed by chains I′′ and Q′′ and G′′′ and O′′′.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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