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Figure 5
Program flow of SEQUENCE SLIDER. Input files provide, along with the diffraction data, the protein sequence, alignment and/or secondary-structure prediction and coordinates from partial solutions. The LLG contribution of each fragment guides selection for further steps. This information is combined to generate most probable hypotheses that are scored based on agreement with previous information. The sequence is modelled onto the trace. The resulting models are refined with either BUSTER, REFMAC5 or phenix.refine and are scored by the Phaser LLG. Once a single hypothesis is clearly favoured, it is fixed and the remaining fragments are re-evaluated in an iterative process until most of the possible sequence match is retrieved. The best-scored models are then submitted to expansion with SHELXE. Steps from external programs are coloured in grey.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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