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Figure 4
Comparison of the structural stability of wild-type and engineered YxaL proteins. The Tm values of the wild-type (WT; black line), T175W/W215G mutant (blue line) and T175W/S213G/W215A mutant (red line) YxaL proteins were measured by thermal shift assay. The line graphs are shown in the left panel and the mean Tm values of the wild-type and mutant YxaL proteins are shown in the table on the right. The individual raw data points of fluorescence displayed for each temperature represent the average of three end-point readings.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 78| Part 1| January 2022| Pages 104-112
https://doi.org/10.1107/S2059798321011724
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