Figure 1
Patterson self-rotation function calculated for the P3 native data set using MOLREP (CCP4): κ = 180°, κ = 120°, κ = 90° and κ = 60°. The self-rotation function describes the crystallographic and noncrystallographic symmetry (NCS) of the contents of the asymmetric unit. The radius of integration used was 27.6 Å and the resolution range was 37–5 Å. The κ = 180° section showed the crystallographic threefold axis perpendicular to the plane of the figure and two sets of three twofold axes in the plane of the figure. These two sets, of different intensities, are rotated 30° with respect to each other. One set describes the D3 point-group noncrystallographic symmetry of the 24 hexamers in the asymmetric unit. The other set describes the 30° rotation of the hexamers with respect to each other in the asymmetric unit, generating the oligomer helix along the z axis. |