X-ray diffraction and in vivo studies reveal the quinary structure of Trypanosoma cruzi nucleoside diphosphate kinase 1: a novel helical oligomer structure

Gomez Barroso, J.A.; Miranda, M.R.; Pereira, C.A.; Garratt, R.C.; Aguilar, C.F.

doi:10.1107/S2059798321011219

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 5
Quinary structure: formation of the oligomer helix. (a) Cartoon representation of the 24 hexamers in the asymmetric unit assembled into the left-handed oligomer helix. (b) Cartoon representation of the 30° hexamer rotation. (c) Connolly surface and electrostatic potential calculated using APBS and PyMOL. An arrow points towards the active-site cleft. (d) Supramolecular association of the left-handed oligomer helices generated by the trigonal crystallographic symmetry along the z axis to form the in vivo granules. (e) Connolly surface and electrostatic distribution of a close-up of the interhexameric interface. The arrow points to the active site. Note the highly positively charged active site for the recognition and binding of highly negative substrates.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 1| January 2022| Pages 30-42

https://doi.org/10.1107/S2059798321011219

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