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Figure 7
Anti-GFP Western blot of parasites expressing TcNDPK1-GFP fusions. (a) SDS–PAGE and densitometry showing correct expression of the transgenes and equal levels of expression among populations. (b) Native PAGE of different populations showing that the mutations do not affect hexamerization. Anti-tubulin antibodies were used as a loading control. Lane 1, N1 (TcNDPK1 without mutation); lane 2; N1-del (TcNDPK1 with Tyr51–Leu63 region deleted); lane 3, N1-ala (TcNDPK1 with Tyr51–Leu63 region replaced by alanines); lane 4, N1-K57R (TcNDPK1 with Lys57 replaced by Arg); lane 5, N1-K57A (TcNDPK1 with Lys57 replaced by Ala); lane 6, N1-P95S (TcNDPK1 with Pro95 replaced by Ser in addition to deletion of the four carboxy-terminal amino acids).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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