X-ray diffraction and in vivo studies reveal the quinary structure of Trypanosoma cruzi nucleoside diphosphate kinase 1: a novel helical oligomer structure

Gomez Barroso, J.A.; Miranda, M.R.; Pereira, C.A.; Garratt, R.C.; Aguilar, C.F.

doi:10.1107/S2059798321011219

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 7
Anti-GFP Western blot of parasites expressing TcNDPK1-GFP fusions. (a) SDS–PAGE and densitometry showing correct expression of the transgenes and equal levels of expression among populations. (b) Native PAGE of different populations showing that the mutations do not affect hexamerization. Anti-tubulin antibodies were used as a loading control. Lane 1, N1 (TcNDPK1 without mutation); lane 2; N1-del (TcNDPK1 with Tyr51–Leu63 region deleted); lane 3, N1-ala (TcNDPK1 with Tyr51–Leu63 region replaced by alanines); lane 4, N1-K57R (TcNDPK1 with Lys57 replaced by Arg); lane 5, N1-K57A (TcNDPK1 with Lys57 replaced by Ala); lane 6, N1-P95S (TcNDPK1 with Pro95 replaced by Ser in addition to deletion of the four carboxy-terminal amino acids).

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 1| January 2022| Pages 30-42

https://doi.org/10.1107/S2059798321011219

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