Figure 8
Fluorescence microscopy of parasites expressing the different mutations. Parasites were fixed, mounted and observed by GFP direct fluorescence. The figure shows one representative field of each parasite population. GFP: fluorescence of the different mutated TcNDPK1 proteins fused to the green fluorescence protein. DIC+GFP: merge of bright-field (DIC) and fluorescence-field (GFP) images. |