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![[Figure 1]](ud5029fig1mag.jpg)
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Figure 1
Reaction schemes and enzyme characterization of SoBDH2 and SrBDH1. The BDHs discussed here preferentially convert the (+)-enantiomers of borneol and isoborneol. SrBDH1 is highly selective for both alcohols and catalyzes the reduction of (+)-camphor. SoBDH2 is highly selective for borneol. PsBDH only shows a slight selectivity for both borneol and isoborneol and is capable of catalyzing the reduction of camphor (Khine et al., 2020  ). (a, b) Reaction schemes of SrBDH1 and SoBDH2 in the enantiospecific oxidation of rac-borneol (a) and rac-isoborneol (b). (c) Reduction of (+)-camphor. (d) SEC-MALS analysis of SrBDH1 (blue) and SoBDH2 (green). For SrBDH1 a single peak is observed consistent with a tetramer (theoretical molecular mass 120 kDa). The first peak in the chromatogram of SoBDH2 corresponds to an octamer (theoretical molecular mass 258 kDa) and the second peak corresponds to a tetramer (theoretical molecular mass 129 kDa). The brown (SrBDH1) and red (SoBDH2) curves are refractive-index signals. (e) Differential scanning fluorometry reveals a significantly lower Tm for SoBDH2 (55.5 ± 0.7°C) compared with SrBDH1 (63.3 ± 1.3°C). |
![[Figure 1]](ud5029fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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