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Figure 1
Apoferritin reconstructions acquired at 200 keV in the presence of 1.7% and 20% glycerol. (a) Fourier shell correlation (FSC) between masked half maps (blue line) and between phase-randomized half maps (black line) from the apoferritin reconstruction in the presence of 20% glycerol. The gray line shows the gold-standard (0.143) correlation value. (b) Reconstruction of apoferritin in the presence of 20% glycerol, colored by local resolution. The right half of the image is a cutaway showing the interior. (c) Apoferritin model (PDB entry 6v21) fitted into the EM density from reconstructions in 20% (blue) and 1.7% (green) glycerol. (d) B-factor plot (Rosenthal & Henderson, 2003BB22) showing the improvement of resolution as a function of the number of particles used for reconstruction in the presence and absence of a high glycerol concentration. (e) Accrued beam-induced motion (per-frame summation) as a function of the number of electrons per Å2 received by the sample. Solid lines: average over all collected movies at a given dose. Colored shades: standard deviation over all movies at a given dose.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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