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Figure 2
Cryo-electron tomography of apoferritin samples acquired in the presence of 1.7% and 20% glycerol. (a) Electron micrographs from sample screening manually blotted for different times. (b) Exemplar micrographs of samples with 1.7% and 20% glycerol. (c) Ice-thickness measurements obtained by electron tomography. Data points are shown as colored dots (horizontally stacked only to aid clarity) overlaid on a gray box that spans the two central quartiles and is crossed by a vertical line at the median. The whiskers span the range of data between the 5th and 95th percentiles. (d) 3D renderings of ice cross-sections from representative micrographs in samples with and without added glycerol, generated by matching an apoferritin density template on the aligned tomograms and placing oriented density surface renderings as markers at high cross-correlation locations.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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