Reconstruction of aldolase at 300 keV in the presence of 20% glycerol. (a) Fourier shell correlation between masked half maps (blue line) and between phase-randomized half maps (black line) from the aldolase density reconstruction in the presence of 20% glycerol. The gray line shows the 0.143 gold-standard correlation value. (b) Density reconstruction of aldolase from data acquired in the presence of 20% glycerol, colored by local resolution. (c) Enlarged segments of aldolase density reconstruction from two areas with different local resolution. PDB entry 5vy5 was aligned with the density and the corresponding atoms are shown inside the mesh.