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Figure 3
Medians and 90% confidence intervals for the sequence-assignment p-value as a function of local resolution for protein-chain test fragments of 20 and 40 amino-acid residues (20aa and 40aa, respectively). (a) shows fragments with sequences matching the reference. Fragments for which the assigned and reference model sequences differ are presented in (b). The dashed line corresponds to a 99.5% one-sided confidence interval estimated for fragments of 20 amino acids with an assigned sequence that differs from the input model sequence. Orange circles depict an outlier that is discussed in the text (the cytoplasmic domain of a transient receptor potential channel; PDB entry 6cv9). The ordinate axes show −log(p-value) for the test fragments; higher values correspond to lower p-values and more reliable sequence assignments.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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