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Figure 9
Fragment of the mTRPC6 cytoplasmic domain at 3.8 Å resolution (a) deposited in the PDB (PDB entry 6cv9/EMDB entry EMD-7637, residues A/258–A/272) and the same fragment rebuilt using AlphaFold2 prediction (Q61143, residues A/264–A/280; the pLDDT for this region exceeds 90). The bottom panels depict the corresponding checkMySequence graphical outputs for the (c) originally deposited and (d) corrected models. Grey bars represent fragments for which the assigned sequence matches the model or has a p-value higher than the threshold (dashed line). The red bars in (c) represent fragments with plausible register errors, including the α-helix shown in (a). The bar plots show −log(p-value) for the test fragments; higher bars correspond to lower p-values and more reliable sequence assignments.

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ISSN: 2059-7983
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