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![[Figure 2]](ij5005fig2mag.jpg)
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Figure 2
Parallel acquisition of tilt series for high-resolution tomography using BISECT. (a) Unlike previous approaches for tomographic data collection that acquire tilt series in a serial manner, BISECT is the first example of the simultaneous acquisition of tilt series, which is capable of collecting data at high magnification without sacrificing resolution. (b) Parallel acquisition of tilt series using a 5 × 5 beam-image shift pattern produces 25 simultaneous tilt series in about 2–3 min per tilt series using a Titan Krios microscope and a K3 camera. |
![[Figure 2]](ij5005fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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