Figure 5
Data-driven CSPT refinement improves ECT/SVA map resolution. (a) Reconstructions obtained using tilt series of rabbit 80S ribosomes (EMPIAR-10064). The original 8.4 Å resolution map (EMDB entry EMD-0529; top) and the 5.6 Å resolution map (EMDB entry EMD-23357) obtained using CSPT (bottom) are shown. (b) Reconstruction of E. coli 70S ribosomes (EMPIAR-10304). The 7.0 Å resolution map (EMDB entry EMD-10211) and the 4.8 Å resolution map (EMDB entry EMD-23358) obtained using CSPT (bottom) are shown (Bouvette et al., 2021). |