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![[Figure 6]](jv5010fig6mag.jpg)
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Figure 6
Conserved water molecules in EcAIII structures. (a) Superposition of WT EcAIII and the T179A mutant (PDB entry 3c17); water molecule w1 is part of the oxyanion hole formed during autoprocessing, while waters w2, w3 and w4 are conserved among almost all variants from the RDM1 series. (b) Superposition of all of the structures determined in this work showing the positions of waters w2, w3 and w4. (c) Superposition of mutants from the present RDM1 series and the complex of EcAIII with L-Asp (PDB entry 2zal); water molecules w2 and w3 superpose with L-Asp, while Trp211 in RDM1-18 occupies the space needed for substrate/product binding (shown in sphere representation). The protein coloring scheme is presented on the right. In all panels, the mutation sites are marked in frames. Water molecules are shown as spheres and hydrogen bonds as dashed lines. |
![[Figure 6]](jv5010fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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