Figure 3
Biochemical characterization. (a) Far-UV CD spectra of RBP (salmon), RBP-CPN (blue) and RBP-CPC (yellow). (b) Normalized tryptophan fluorescence at a 280 nm excitation wavelength of RBP (salmon), RBP-CPN (blue) and RBP-CPC (yellow). (c) DSC endotherms of RBP (salmon), RBP-CPN (blue) and RBP-CPC (yellow); sample concentrations of 0.5, 1 and 5 mg ml−1 are shown as solid, dashed and dotted lines, respectively. (d) SEC-MALS analysis of RBP-CPN (blue) and RBP-CPC (yellow) at different concentrations. The elution profile is plotted as the relative differential refractive index against the retention time. Sample concentrations of 0.5, 1 and 5 mg ml−1 are shown as solid, dashed and dotted lines, respectively. Molar-mass determinations for peak regions are plotted as gray dots. |