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![[Figure 2]](ih5005fig2mag.jpg)
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Figure 2
Images from the VitroJet cameras to assess the sample pickup and deposition process and the resulting estimation of ice thickness. (a) Image from the pipette camera after droplet pickup. The sample is presented as a half sphere at the tip of the pipette, after which the solid pin dips into the sample. Upon retraction, a sample volume of approximately 0.5 nl is located on the front face of the pin. The pipette tip is situated in the temperature-controlled wall of the climate chamber. (b) Image from the grid camera to monitor sample deposition by pin printing. In this example, the pin started in the center of the field of view and spiraled outwards to cover an area with a diameter of 800 µm on a 200 mesh R2/1 grid. The intensity of the deposited layer indicates the thickness of the layer. (c) Color overlay of the estimated ice thickness for each individual hole based on the grid-camera images during deposition. (d) Histogram of the estimated thicknesses for the holes in the perforated foil, with a total of 12 093 holes detected in the squares where the sample was deposited. |
![[Figure 2]](ih5005fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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