Structural determination and modeling of ciliary microtubules

This review describes how electron-microscopy methods have helped to reveal the structure of the ciliary axoneme.

VitroJet: new features and case studies

This paper presents recent technical improvements to the VitroJet and the benefits that it brings to the cryo-EM workflow, as well as a wide variety of case studies. This illustrates the advancement of the VitroJet into an instrument that enables accurate control and reproducibility, demonstrating its suitability for time-efficient cryo-EM structure determination.

Tomo Live: an on-the-fly reconstruction pipeline to judge data quality for cryo-electron tomography workflows

To simplify the cryo-electron tomography workflow and make this technique more accessible to all researchers, the Tomo Live software has been developed, which performs on-the-fly reconstruction of tilt series, enabling real-time data-quality monitoring, curation and export.

EMinsight: a tool to capture cryoEM microscope configuration and experimental outcomes for analysis and deposition

EMinsight is a Python-based tool for systematically mining metadata from single-particle analysis cryoEM experiments. The capture and analysis of metadata facilitates the assessment of instrument performance, provides concise reporting of experiment performance and sample quality by analysing preprocessing results, and gathers metadata for deposition. It is envisaged that this approach will benefit the microscope operator, facility managers, database developers and users.

AlphaFold-assisted structure determination of a bacterial protein of unknown function using X-ray and electron crystallography

The structure determination of a small protein of unknown function by molecular replacement using a search model predicted by new machine-learning methods is reported. Notably, the approach was successful using electron diffraction data collected from a protein microcrystal, highlighting a potentially important new route for structure determination.

Efficient in situ screening of and data collection from microcrystals in crystallization plates

A sample- and time-efficient method to obtain serial crystallography data from batch-grown microcrystals dispensed as drops on a 96-well crystallization plate is described. This offers a versatile method to obtain low-dose room-temperature structures and guide the optimization of microcrystallization for synchrotron and XFEL serial crystallography experiments.

Mononuclear binding and catalytic activity of europium(III) and gadolinium(III) at the active site of the model metalloenzyme phosphotriesterase

The crystal structures of europium(III)- and gadolinium(III)-bound phosphotriesterase are presented along with esterase activity data for the lanthanide-bound enzymes.