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![[Figure 4]](ih5005fig4_combined_mag.jpg)
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Figure 4
Case studies of the VitroJet with a variety of samples. (a) Representative micrographs for use cases with different dimensions. I: membrane proteins with a diameter of 10 nm reconstructed to a resolution of 2.7 Å. II: nucleosome micrograph with a size of 12 nm, which is representative, reconstructed to 3.0 Å resolution. III: fatty-acid synthase (FAS) representative micrograph with a diameter of 27 nm, reconstructed to 3.3 Å resolution. IV: micrograph of tick-borne encephalitis virus with a size of 50 nm. V: representative micrograph of LNPs vitrified with the VitroJet with diameters ranging from 60 to 100 nm. VI: iDPC–STEM micrograph of Tobacco mosaic virus (TMV) samples with vitreous ice micrograph at CSA = 2.0 mrad. VII: micrograph of purified bacteriophage FJy-3 with a capsid diameter of approximately 150 nm. (b) Selected 2D classes of FAS exclusively show side views. Fourier shell correlation after nonuniform refinement in cryoSPARC, a local resolution map and a slab of local resolution map are shown. The slab of the FAS center with colored reconstructed map and PDB entry 6ta1 shows clear side-chain features. (c) Selected 2D classes of the TMV sample reconstructed to an FSC(0.143) resolution of 5.4 Å. The real-space top and side views of the reconstructed map show the clear secondary-structure features that are expected at this resolution. |
![[Figure 4]](ih5005fig4_combined.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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