Figure 1
Para-nitrophenyl butyrate hydrolysis assay. Para-nitrophenol absorbance at 405 nm, measured over 5 min, with buffer consisting of 20 mM Tris, 100 mM NaCl, 2.5%(v/v) methanol pH 8.5 (magenta) and with buffer with 100 µM ZnCl2 (black), with 100 nM dialysed protein (cyan) and with 100 nM dialysed protein and 100 µM ZnCl2 (green) (N = 3). |