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![[Figure 3]](qv5004fig3mag.jpg)
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Figure 3
Tomo Live workflow. (a) Diagram of the processing pipeline. (b, c, d) Stack cleaner showing histogram correlation maps of (b) a clean stack without non-usable tilt images and (c) a stack with four non-usable tilt images at high tilt angles (d). (e) Pair-alignment workflow whereby cross-correlation between tilt image i and tilt image i + 1 is computed; the cross-correlation peak is found to estimate shifts in X and Y. (f, g) Tomogram positioning. (f) The fitting plan of the 3D positions with outliers shown in red. (g) Illustration of tomogram-orientation correction in the XZ plane. (h, i) CTF determination. (h) Division of the image into patches parallel to the tilt axis (represented by white patches). (i) Representative CTF curve with fitting. (j, k) 3D reconstruction. (j) Contrast function of a binned-down reconstruction. (k) Cross section of a reconstruction where the thickness was determined automatically. |
![[Figure 3]](qv5004fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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