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![[Figure 7]](qv5004fig7mag.jpg)
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Figure 7
CTF estimation by Tomo Live and CTFFIND4. (a) Estimated defocus by Tomo Live minus estimated defocus by CTFFIND4 for purified KLH and yeast lamellae (left) for all data points and (right) zoomed-in. Each point denotes one defocus estimate in one tilt image. The color of the points depicts the tilt angle at which the image was recorded. Defocus estimates with estimated resolution accuracy >30 Å for CTFFIND4 were excluded. The same results as shown in (a) shown per tilt angle for (b) KLH and (c, d) for yeast lamellae: (c) all data points and (d) zoomed-in. (e, f) The difference in defocus estimates for higher tilt angles compared with the estimate for the zero-tilt image of the same tilt series for (e) Tomo Live and (f) CTFFIND4 for KLH. (g, h) The difference in defocus estimates for higher tilt angles compared with the estimate for the zero-tilt image of the same tilt series for (g) Tomo Live and (h) CTFFIND4 for yeast lamella. Each point denotes one defocus estimate in one tilt image. In all panels, the black line shows the median difference between the two estimates and the gray-shaded area shows the interquartile range. |
![[Figure 7]](qv5004fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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